What critical observation led Ivanovsky to question the nature of the pathogen or the filter itself?

Answer

The sap that passed through the filter retained its ability to cause mosaic disease

The sap remained infectious after passing through the filter designed to stop all known bacteria. This contradicted the expectation that the filtration process should have removed the causative agent.

What critical observation led Ivanovsky to question the nature of the pathogen or the filter itself?
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